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Haishan Zeng, PhD, University of British Columbia, Vancouver, BC. Ultrafast femtosecond (fs) laser pulse with intense light energies concentrated in a time period of as short as one millionth of a billionth second (10-15 s) creates instant super high light power of hundreds of kilowatts. When focused to a biological target such as a cell, this high intensity light pulse generates non-linear optical effects such as two-photon excitation fluorescence (TPEF), second- and third- harmonic generation (SGH and TGH), and two-photon absorption induced heating. TPEF, SGH, and TGH can be utilized to perform microscopic tissue imaging with much better tissue penetration and higher signal-to-noise (S/N) ratio as compared to conventional one-photon based imaging modalities, while two-photon absorption induced heating can be explored for therapy. The advantage of two-photon absorption over conventional lasers or other light sources is that extremely precise and spatially confined energy delivery can be achieved, allowing us to specifically kill diseased and/or cancer cells while leaving the surrounding normal cells unaltered. Our objectives are (1) to develop an ultrafast laser-based multi-photon imaging and spectroscopy system for non-invasive skin diagnosis at the cellular level, and (2) to treat the diseased skin cells that are detected by this system using the same ultrafast laser at higher power settings under the guidance of the system's imaging component. If successful, a "see and treat" system will be realized that will allow us to diagnose and treat various skin diseases noninvasively and on a cell by cell basis. Peer-reviewed publications supported by the Canadian Dermatology Foundation 1. Lau, D.P., Huang, Z., Lui, H., Man, C.S., Berean, K., Morrison, M.D., Zeng, H.. Raman spectroscopy for optical diagnosis in normal and cancerous tissue of the nasopharynx - preliminary findings Lasers in Medicine and Surgery 32(3), 210-214 (2003). 2. Huang Z, McWilliams A, Lui H, McLean DI, Lam S, Zeng H. Near-infrared Raman spectroscopy for optical diagnosis of lung cancer. Int J Cancer 107(6):1047-52. (2003). 3. Huang, Z., McWilliams, A., Lam, S., English, J. McLean, D.I., Lui, H. Zeng, H. Effect of Formalin Fixation on the Near-Infrared Raman Spectroscopy of Human Bronchial Tissues Intl. J. of Oncology, 23: 649-656 (2003). 4. Bernardo O, Tang L, LUI H, Shapiro J. Topical nitrogen mustard in the treatment of alopecia areata: A bilateral comparison study. J Am Acad Dermatol 2003; 291-294. 5. Shum DT, LUI H, Martinka M, Bernardo O, Shapiro J. Computerized morphometry and three-dimensional image reconstruction in the evaluation of scalp biopsy from patients with non-cicatricial alopecias. Brit J Dermatol 2003; 148:1-7. 6. Tang L, Bernardo O, Bolduc C, LUI H. Shapiro J. The expression of insulin-like growth factor I in follicular dermal papillae correlates with therapeutic efficacy of finasteride in adrogenetic alopecia. J Am Acad Dermatol 2003; 229-233. 7. Tang L, Cao L, Bernardo O, Chen Y, Sundberg, JP, LUI H, Chung S, Shapiro J. Topical mechlorethamine restores autoimmune-arrested follicular activity in mice with alopecia areata-like disease by targeting infiltrated lymphocytes. J Invest Dermatol 2003; 120: 400-406. 8. Tang L, Cao K, Pelech S, LUI H, Shapiro J. Cytokines and signal transduction pathways mediated by anthralin in alopecia areata-affected Dundee experimental balding rats. J Invest Dermatol Symp Proc 2003;8:87-90. 9. Tang L, LUI H, Sundberg J, Shapiro J. Old wine in new bottles: Reviving old therapies for alopecia areata using rodent models. J Invest Dermatol Proceedings. 2003; 8:212-6. 10. Tang, LUI H, Sundberg JP, Bissonnette R, McLean DI, Shapiro J. Restoration of hair growth with topical diphencyprone in murine and rate models of alopecia areata. J Am Acad Dermatol 2003; 49:1013-1019. 11. Huang, Z, Lui H, Chen MXK, McLean DI, Zeng, H.: Raman Spectroscopy of In Vivo Cutaneous Melanin. J. of Biomedical Optics, 9(6):1198-1205, 2004. 12. Hamzavi, I.,Jain, H., McLean, D.I., Shapiro, J., Zeng, H., Lui, H.: Parametric Modelling of Narrow Band UV-B Phototherapy for Vitiligo Using a Novel Quantitative Tool, Arch. Dermatol. 140: 677-683, 2004. 13. Tang L, Cao L, Sundberg JP, LUI H, Shapiro J. Restoration of hair growth in mice with an alopecia areata-like disease using topical anthralin. Exp Dermatol 2004; 13:5-10. ©2008 |
