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Jianhua Zhao PhD, University of British Columbia, Vancouver, BC. Melanin, being a universal natural pigment in the skin, is important in providing protection to the skin from ultraviolet light. It is also believed to provide an important role in malignant pigmented skin lesons. Melanin has been studied extensively by a wide variety of techniques, however, most of the studies have been limited to measuring melanin samples outside of the body. Measuring melanin directly in the skin poses a huge challenge, partly because it is so efficient at absorbing light. Recently by utilizing a new medical tool called a near-infrared (NIR) Raman spectrometer, we have found that natural and synthetic melanin exhibits significant and measurable optical signals known as Raman and autofluorescence. Pigmented lesions have higher NIR autofluorescences than normal skin. This project is designed to further characterize and systematically quantify melanin in pigmented skin disorders by exploiting these new findings and new technologies. It will provide a completely novel and potentially powerful modality for measuring cutaneous melanin directly in the skin and for understanding the underlying mechanisms of melanin in various pigmented skin disorders. Publications Supported by the Canadian Dermatology Foundation (2003-07) 1. Lau, D.P., Huang, Z., Lui, H., Man, C.S., Berean, K., Morrison, M.D., Zeng, H.. Raman spectroscopy for optical diagnosis in normal and cancerous tissue of the nasopharynx - preliminary findings Lasers in Medicine and Surgery 32(3), 210-214 2. Huang Z, McWilliams A, Lui H, McLean DI, Lam S, Zeng H. Near-infrared Raman spectroscopy for optical diagnosis of lung cancer. Int J Cancer 107(6):1047-52. (2003). 3. Huang, Z., McWilliams, A., Lam, S., English, J. McLean, D.I., Lui, H. Zeng, H. Effect of Formalin Fixation on the Near-Infrared Raman Spectroscopy of Human Bronchial Tissues Intl. J. of Oncology, 23: 649-656 (2003). 4. Bernardo O, Tang L, LUI H, Shapiro J. Topical nitrogen mustard in the treatment of alopecia areata: A bilateral comparison study. J Am Acad Dermatol 2003; 291-294. 5. Shum DT, LUI H, Martinka M, Bernardo O, Shapiro J. Computerized morphometry and three-dimensional image reconstruction in the evaluation of scalp biopsy from patients with non-cicatricial alopecias. Brit J Dermatol 2003; 148:1-7. 6. Tang L, Bernardo O, Bolduc C, LUI H. Shapiro J. The expression of insulin-like growth factor I in follicular dermal papillae correlates with therapeutic efficacy of finasteride in adrogenetic alopecia. J Am Acad Dermatol 2003; 229-233. 7. Tang L, Cao L, Bernardo O, Chen Y, Sundberg, JP, LUI H, Chung S, Shapiro J. Topical mechlorethamine restores autoimmune-arrested follicular activity in mice with alopecia areata-like disease by targeting infiltrated lymphocytes. J Invest Dermatol 2003; 120: 400-406. 8. Tang L, Cao K, Pelech S, LUI H, Shapiro J. Cytokines and signal transduction pathways mediated by anthralin in alopecia areata-affected Dundee experimental balding rats. J Invest Dermatol Symp Proc 2003;8:87-90. 9. Tang L, LUI H, Sundberg J, Shapiro J. Old wine in new bottles: Reviving old therapies for alopecia areata using rodent models. J Invest Dermatol Proceedings. 2003; 8:212-6. 10. Tang, LUI H, Sundberg JP, Bissonnette R, McLean DI, Shapiro J. Restoration of hair growth with topical diphencyprone in murine and rate models of alopecia areata. J Am Acad Dermatol 2003; 49:1013-1019. 11. Huang, Z, Lui H, Chen MXK, McLean DI, Zeng, H.: Raman Spectroscopy of In Vivo Cutaneous Melanin. J. of Biomedical Optics, 9(6):1198-1205, 2004. 12. Hamzavi, I.,Jain, H., McLean, D.I., Shapiro, J., Zeng, H., Lui, H.: Parametric Modelling of Narrow Band UV-B Phototherapy for Vitiligo Using a Novel Quantitative Tool, Arch. Dermatol. 140: 677-683, 2004. 13. Tang L, Cao L, Sundberg JP, LUI H, Shapiro J. Restoration of hair growth in mice with an alopecia areata-like disease using topical anthralin. Exp Dermatol 2004; 13:5-10 14. Huang, Z, Lui, H., McLean, DI, Korbelik, Zeng, H: Raman Spectroscopy in Combination with Background Near-Infrared Autofluorescence Enhances the In Vivo Assessment of Malignant Tissues. Photochemistry and Photobiology, 81: 1219-1226, 2005 15. Lau DP, Huang Z, Lui H, Anderson DW, Berean K, Morrison MD, Shen L, Zeng H. Raman spectroscopy for optical diagnosis in the larynx: preliminary findings. Lasers Surg Med. 37(3):192-200. (2005). 16. Hamzavi, I.,Shiff, N., Martinka, M., Huang, Z., McLean, DI., Zeng, H., Lui, H.: Spectroscopic Assessment of Dermal Melanin using Blue Vitiligo as an in vivo Model. Photodermatology, Photoimmunology & Photomedicine, 22:46-51, 2006. 17. Short, M.A., Lui, H., McLean, D.I., Zeng, H., Alajlan, A., Chen, M.X.: Demonstrating Changes in Nuclei and Peritumoral Collagen within Nodular Basal Cell Carcinomas Via Confocal Micro-Raman Spectroscopy, J of Biomedical Optics, 11: 034004, 2006. 18. Huang Z, Zeng H, Hamzavi I, Alajlan A, Tan E, McLean DI, LUI H. Cutaneous Melanin Exhibits Fluorescence Emission Under Near-Infrared Light Excitation. J. of Biomedical Optics, 11: 034010, 2006. 19. Markhvida, I, Tchvialeva, L, Lee, TK, and Zeng, H.: The Influence of Geometry on Polychromatic Speckle Contrast, Journal of OSA A, 24 (1), 93-97 (2006). 20. Chen, R., Huang, Z., Lui, H., Hamzavi, I., McLean, D.I., Xie, S., Zeng, H.: Monte Carlo simulation of cutaneous reflectance and fluorescence measurements —— the effect of melanin contents and localization, Journal of photochemistry and photobiology B: Biology, 86 (3): 219-226 (2007) 21. J. Zhao, H. Lui, D. I. McLean, and H. Zeng, "Automated fluorescence background subtraction in biomedical Raman spectra", Applied Spectroscopy 61, 1225 (2007) 22. J. Zhao, H. Lui, D. I. McLean, and H. Zeng, "Towards instrument independent quantitative measurement of fluorescence intensity in fiber optic spectrometer system", Applied Optics 46, 7132-7140 (2007) 23 J. Zhao, H. Lui, D. I. McLean, and H. Zeng, "Integrated real-time Raman system for clinical in vivo skin analysis", Skin Research and Technology, accepted, 2007 (in press) 24. Zeng, H, Zhao J, Short M, McLean D, Lam S, McWilliams A, and LUI H. Raman Spectroscopy for in vivo Tissue Analysis and Diagnosis – from Instrument Development to Clinical Applications. J Innovative Optical Health Research 2007 (in press). ©2008 |
